Zymography Methods

Definition:

Zymography is used to measure the activity of enzymes and is widely applied in enzyme research, metabolic pathway analysis, and drug screening. Samples are run in a gel containing gelatin under non-reducing conditions. The gel is then incubated under enzyme activity conditions, and after staining with Coomassie Brilliant Blue, areas of gelatin depletion are used as an indicator of enzyme activity. This method provides an intuitive reflection of the enzyme's action on its substrate.

References:

[1]. Vandooren J, et al. Zymography methods for visualizing hydrolytic enzymes. Nat Methods. 2013 Mar;10(3):211-20. [Content Brief]

Biomedical Dictionary

The Biomedical Dictionary is a comprehensive and professional collection of biological academic terms and subject datas. All explanations are supported by authoritative books or high impact factor literatures, and you can acquire accurate explanations of the biomedical terms you want to know.

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z #

Name
Email *

	Sorry, but the email address you supplied was invalid.