Calcitonin Gene Related Peptide (CGRP) II, rat

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Calcitonin Gene Related Peptide (CGRP) II, rat, a CGRP receptor activator, is a potent and long-lasting vasodilator. Calcitonin Gene Related Peptide (CGRP) II can be used in the research of cardiovascular diseases.

For research use only. We do not sell to patients.

Calcitonin Gene Related Peptide (CGRP) II, rat Chemical Structure

CAS No. : 99889-63-1

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Other Forms of Calcitonin Gene Related Peptide (CGRP) II, rat:

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1 Publications Citing Use of MCE Calcitonin Gene Related Peptide (CGRP) II, rat

•Res Sq. 2024 May 10.

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Calcitonin Gene Related Peptide (CGRP) II, rat induces vasodilator effects in small-diameter pig left anterior descending (LAD) coronary arteries with an EC₅₀ value of 0.56 nM^[1].
Calcitonin Gene Related Peptide (CGRP) II, rat relaxes the spontaneous tone in isolated internal anal sphincter (IAS) strips, with an EC₅₀ value of 83 μM^[2].
Calcitonin Gene Related Peptide (CGRP) II, rat (rβCGRP) inhibits [¹²⁵I]hαCGRP binding (IC₅₀: 7 nM), and stimulates cAMP accumulation (EC₅₀: 0.56 nM) in the cells co-expressing mRAMP1 (mouse receptor-activity-modifying-protein 1) and rCRLR (rat calcitonin receptor-like receptor)^[3].
Calcitonin Gene Related Peptide (CGRP) II, rat (rat-βCGRP, 0.01-100 nM) induces endothelium-independent relaxations in the coronary arteries from male and female Sprague-Dawley rats, with an IC₅₀ value of 2.8 nM approximately^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Calcitonin Gene Related Peptide (CGRP) II, rat (rat-βCGRP, bolus injection, 0.3 μg/kg, at 10 min intervals) induces hypotension and vasodilatation in Male Sprague-Dawley rats^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague-Dawley rats^[5].
Dosage:	0.01 ng/kg-3 μg/kg (100 μL), succeeded by a 150 μL isotonic saline flush, at 10 min intervals.
Administration:	A bolus injection
Result:	Induced PA (pial artery) dilatation and increased in LCBF_Flux (local cortical cerebral blood flow).

Molecular Weight

3805.31

Formula

C₁₆₃H₂₆₇N₅₁O₅₀S₂

CAS No.

99889-63-1

Sequence

Ser-Cys-Asn-Thr-Ala-Thr-Cys-Val-Thr-His-Arg-Leu-Ala-Gly-Leu-Leu-Ser-Arg-Ser-Gly-Gly-Val-Val-Lys-Asp-Asn-Phe-Val-Pro-Thr-Asn-Val-Gly-Ser-Lys-Ala-Phe-NH2 (Disulfide bridge: Cys2-Cys7)

Sequence Shortening

SCNTATCVTHRLAGLLSRSGGVVKDNFVPTNVGSKAF-NH2 (Disulfide bridge: Cys2-Cys7)

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

H₂O

Peptide Solubility and Storage Guidelines:

1. Calculate the length of the peptide.

2. Calculate the overall charge of the entire peptide according to the following table:

	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3. Recommended solution:

Overall charge of peptide	Details
Negative (<0)	1. Try to dissolve the peptide in water first. 2. If water fails, add NH₄OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)	1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. D Wu, et al. Development and potential of non-peptide antagonists for calcitonin-gene-related peptide (CGRP) receptors: evidence for CGRP receptor heterogeneity. Biochem Soc Trans. 2002 Aug;30(4):468-73. [Content Brief]

[2]. F M Wisskirchen, et al. CGRP(2) receptor in the internal anal sphincter of the rat: implications for CGRP receptor classification. Br J Pharmacol. 2000 May;130(2):464-70. [Content Brief]

[3]. K Husmann, et al. Mouse receptor-activity-modifying proteins 1, -2 and -3: amino acid sequence, expression and function. Mol Cell Endocrinol. 2000 Apr 25;162(1-2):35-43. [Content Brief]

[4]. M Sheykhzade, et al. Characterization of calcitonin gene-related peptide (CGRP) receptors in intramural coronary arteries from male and female Sprague Dawley rats. Br J Pharmacol. 1998 Apr;123(7):1464-70. [Content Brief]

[5]. Kenneth A Petersen, et al. Inhibitory effect of BIBN4096BS on cephalic vasodilatation induced by CGRP or transcranial electrical stimulation in the rat. Br J Pharmacol. 2004 Nov;143(6):697-704. [Content Brief]