Cross-linked dextran G 50, medium 

Cross-linked dextran G 50, medium is a gel filtration medium. Cross-linked dextran G 50, medium can be used in gel permeation chromatography for fractionation of the glycopeptide mixture (Sphere protein separation range: 1.5K-30K Da; Polysaccharide separation range: 500-10K Da). Cross-linked dextran G 50, medium are microspheres with an average particle size of D50 = 220-250 μm^[1][2].

Cross-linked dextran G 50, medium can be used for isolation and purification of protected peptides with certain molecule range.
Methods for Cross-linked dextran G 50, medium instructment:
1. Gel swelling: Swell the G-50 gel in a hexamethylphosphoramide (HMPA) solvent containing 5% water at 50°C for 16 hours.
2. Degassing: Degas the swollen gel to remove dissolved air.
3. Column loading: Load the degassed G-50 gel into the chromatographic column with an inner diameter of 2.5 cm or 5.0 cm.
4. Sample loading: Load the sample to be purified onto the packed G-50 column.
5. Elution: Elute the chromatographic column with HMPA-5% aqueous solution and elute at a flow rate of 7.5 mL/h or 20 mL/h. Monitor the UV absorbance of the eluent at 280 nm.
6. Collect: Collect the eluent of the target peptide for subsequent purifications.
7. Remove HMPA: Since HMPA cannot be removed by evaporation, the peptide can be separated from HMPA by gel filtration using a G-10 gel column and DMF solvent.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9048-71-9

[Cross-linked dextran G 50, medium]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Galpin IJ, et, al. Gel filtration of protected peptides on sephadex G-50 in hexamethylphosphoramide containing 5% water. J Chromatogr. 1975 Mar 19;106(1):125-9.  [Content Brief]

  [2]. Meezan E, et, al. Comparative studies on the carbohydrate-containing membrane components of normal and virus-transformed mouse fibroblasts. II. Separation of glycoproteins and glycopeptides by sephadex chromatography. Biochemistry. 1969 Jun;8(6):2518-24.  [Content Brief]