DAPI (dihydrochloride) (solution) (Synonyms: 4',6-Diamidino-2-phenylindole dihydrochloride (solution))

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DAPI dihydrochloride (4',6-Diamidino-2-phenylindole dihydrochloride) (solution) is a DAPI dye. DAPI is a fluorescent dye that can bind strongly to DNA. DAPI binds to the A/T base pairs in the minor groove of double-stranded DNA (1 DAPI molecule can occupy 3 base pairs), which amplifies the fluorescence intensity by about 20 times. By observing the fluorescence intensity, DNA can be quantified. DAPI can also penetrate intact cell membranes and is often used to stain living and fixed cells.

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DAPI (dihydrochloride) (solution) Chemical Structure

CAS No. : 28718-90-3

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Solvent
500 μg (28.55 μM * 50 mL in DDH2O)		Ask For Quote & Lead Time
Solvent
5 mg (28.55 μM * 500 mL in DDH2O)		Ask For Quote & Lead Time

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58 Publications Citing Use of MCE DAPI (dihydrochloride) (solution)

: DAPI (dihydrochloride) (solution) purchased from MedChemExpress. Usage Cited in: Pharmaceuticals. 2022 Jun 6;15(6):721.; The nuclei are counterstained with DAPI (10 μM) in the dark for 10 min at room temperature. Fluorescence is monitored using confocal microscope, and foci of phospho-H2A.X was quantified using ImageJ software.

: DAPI (dihydrochloride) (solution) purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2021 Jul;42(7):1111-1123. [Abstract]; Mice intestine tissues sections were incubated with a TUNEL reaction mixture for 1 h at 37 °C in the dark. Next, the sections are stained with 0.1 μg/mL DAPI.

: DAPI (dihydrochloride) (solution) purchased from MedChemExpress. Usage Cited in: Oncotargets Ther. 2020 Oct 6;13:9941-9952. [Abstract]; Human GC MGC-803 and NCI-N87 cells are seeded into 24-well plates with glass slide for 24h in RPMI-1640 medium. And 0.1% DAPI staining is performed against light for 30 minutes.

: DAPI (dihydrochloride) (solution) purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2019 Sep 15;96:468-479. [Abstract]; CLSM observations of tested Cy5-nanocomplexes incubated with RAW 264.7 macrophages show the fluorescent distributions of DAPI, nanocomplexes, and ROS (DCFH-DA) in the presence and absence NIR treatment.

: DAPI (dihydrochloride) (solution) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2020 Mar;68:153146. [Abstract]; MC3T3-E1 cells incubated with 1 μg/ml DAPI dihydrochloride for 5 min and observed them under a fluorescence microscope. Effects of sweroside on ER-α and GPR30 expressions by immunofluorescence, white and yellow arrows mean target protein distribution in the cell nuclei and membrane, respectively.

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Description	DAPI dihydrochloride (4',6-Diamidino-2-phenylindole dihydrochloride) (solution) is a DAPI dye. DAPI is a fluorescent dye that can bind strongly to DNA. DAPI binds to the A/T base pairs in the minor groove of double-stranded DNA (1 DAPI molecule can occupy 3 base pairs), which amplifies the fluorescence intensity by about 20 times. By observing the fluorescence intensity, DNA can be quantified. DAPI can also penetrate intact cell membranes and is often used to stain living and fixed cells^[1].
Molecular Weight	350.25
Formula	C₁₆H₁₇Cl₂N₅
CAS No.	28718-90-3
SMILES	N=C(C1=CC2=C(C=C1)C=C(C3=CC=C(C(N)=N)C=C3)N2)N.[H]Cl.[H]Cl
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation	Data Sheet (267 KB) Handling Instructions (2659 KB)
References	[1]. Kapuscinski J. DAPI: a DNA-specific fluorescent probe[J]. Biotechnic & histochemistry, 1995, 70(5): 220-233. [Content Brief]

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Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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DAPI (dihydrochloride) (solution) (Synonyms: 4',6-Diamidino-2-phenylindole dihydrochloride (solution))

Other Forms of DAPI (dihydrochloride) (solution):

DAPI (dihydrochloride) (solution) Related Antibodies

58 Publications Citing Use of MCE DAPI (dihydrochloride) (solution)

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review