ddGTP trisodium  (Synonyms: 2′,3′-Dideoxyguanosine 5′-triphosphate trisodium)

ddGTP (2′,3′-Dideoxyguanosine 5′-triphosphate) trisodium is one of 2',3'-dideoxyribonucleoside 5'-triphosphates (ddNTPs) that acts as chain-elongating inhibitor of DNA polymerase for DNA sequencing. ddGTP trisodium acts as an inhibitor or a substrate for DNA polymerase α^[1][2][3].

ddGTP trisodium inhibits the formation of both negative and positive DNA strands^[1]. ddGTP trisodium inhibits DNA polymerase α activity in the presence of Mn^[2+] at low concentrations (<1 μM) ^[2]. ddGTP trisodium competitively inhibits the incorporation of [3H]dGTP into the DNA^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

557.13

C₁₀H₁₃N₅Na₃O₁₂P₃

Liquid

Colorless to light yellow

O=[P](O[P](O[P](O)(O[Na])=O)(O[Na])=O)(O[Na])OC[C@@H]1CC[C@H](N2C(NC(N)=NC3=O)=C3N=C2)O1

Room temperature in continental US; may vary elsewhere.

Solution, -20°C, 2 years

• [1]. Debyser Z, et al. Kinetics of inhibition of endogenous human immunodeficiency virus type 1 reverse transcription by 2',3'-dideoxynucleoside 5'-triphosphate, tetrahydroimidazo-[4,5,1-jk][1,4]-benzodiazepin-2(1H)-thion e, and 1-[(2-hydroxyethoxy)methyl]-6-(phenylthio)thymine derivatives. J Biol Chem. 1992 Jun 15;267(17):11769-76.  [Content Brief]

  [2]. Ono K, et al. Utilization of 2',3'-dideoxyguanosine 5'-triphosphate as an inhibitor and substrate for DNA polymerase alpha. Biomed Pharmacother. 1990;44(2):115-21.  [Content Brief]

  [3]. Z G Chidgeavadze, et al. 3'-Fluoro-2',3'-dideoxyribonucleoside 5'-triphosphates: terminators of DNA synthesis. FEBS Lett. 1985 Apr 22;183(2):275-8.  [Content Brief]