DPNB-ABT594 

DPNB-ABT594 is a nitrobenzyl-caged ABT594 (HY-14316A) and activates nAChRs containing the α4β2 subunits with good selectivity than the α7 subunit. DPNB-ABT594 can be used to map the distribution of nAChRs on neurons of the medial habenula (MHb) and helps to gain a deeper understanding of the nAChR‐mediated Ca²⁺ signalling in the MHb^[1].

Target: α4β2 nAChR; α7 nAChR^[1]

DPNB‐ABT594 (20 μM) exhibits large inward currents and Ca²⁺ transients in response to laser uncaging at 410 nm at the soma, which increases linearly with energy dosage as expected for one‐photon excitation (1 mW, 0.5-3 ms)^[1].
DPNB‐ABT594 is suitable for one- or two-photon photolysis, it evokes large inward currents and Ca²⁺ transients on cell bodies and dendrites of medial habenular neurons in mouse brain slices, following exposure to 410 nm light^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

672.16

C₃₁H₄₆ClN₃O₁₁

ClC1=NC=C(OC[C@@H]2N(C(C3=CC(OCCOCCOCCOC)=C(OCCOCCOCCOC)C=C3[N+]([O-])=O)C)CC2)C=C1

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Stefan Passlick, et al. Optical Probing of Acetylcholine Receptors on Neurons in the Medial Habenula With a Novel Caged Nicotine Drug Analogue.J Physiol. 2018 Nov;596(22):5307-5318.  [Content Brief]