Elastase, Human leukocytes 

Elastase, Human leukocytes is a serine protease present in the nitrogen-loving granules of neutrophils. Elastase, Human leukocytes potential substrates include almost all components of the extracellular matrix, as well as a variety of proteins such as coagulation factors, complement, immunoglobulins and cytokines. It has a strong proteolytic function and participates in the pathogenesis of inflammatory tissue damage^[1][2].

Elastase, Human leukocytes hydrolysis IL-36Ra experiment^[2]

(1) Cells were grown in serum-free medium or Opti-MEM medium to prevent any inhibition of active proteases. After isolation, neutrophils were immediately stimulated with 100 ng/mL PMA at 37°C for 1 hour, then centrifuged to collect the cells and supernatant.
(2) 1-2 μg of SUMO-tagged IL-36Ra was incubated with the supernatant from primary cells at 37°C for 1 hour, with or without protease inhibitors (IAA 50 μM, EDTA 500 μM, PMSF 1 mM, 1x Roche protease inhibitor mixture, α1-antitrypsin 1 mg/ml).
(3) Incubate with Elastase, Human leukocytes at 37°C for 5 hours.
(4) Analyze using SDS-PAGE or Western Blotting with a monoclonal anti-IL-36Ra antibody.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9004-06-2

Solid

White to off-white

[Elastase, Human leukocytes]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. W L Lee, et al. Leukocyte elastase: physiological functions and role in acute lung injury. Am J Respir Crit Care Med. 2001 Sep 1;164(5):896-904.  [Content Brief]

  [2]. Tom Macleod, et al. Neutrophil Elastase-mediated proteolysis activates the anti-inflammatory cytokine IL-36 Receptor antagonist. Sci Rep. 2016 Apr 22:6:24880.  [Content Brief]