Estrogen receptor-IN-1

Name: Estrogen receptor-IN-1
Brand: MedChemExpress
SKU: HY-150601
Rating: 4.5 (1 reviews)

Cat. No.: HY-150601 Purity: 99.87%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

Estrogen receptor-IN-1 (compound 16) is a potent estrogen receptor (ER) inhibitor with IC₅₀s of 13, 5µM for ERα and Erβ, respectively.

For research use only. We do not sell to patients.

Estrogen receptor-IN-1 Chemical Structure

CAS No. : 74027-99-9

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Estrogen receptor-IN-1

•Cell Rep. 2023 Dec 1;42(12):113504. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Estrogen receptor-IN-1 (compound 16) is a potent estrogen receptor (ER) inhibitor with IC₅₀s of 13, 5µM for ERα and Erβ, respectively^[1].

IC₅₀ & Target^[1]

ERα

13 μM (IC₅₀)

ERβ

5 μM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	> 30 μM Compound: 16	Agonist activity at human Gal4-fused ERalpha expressed in HEK293 cells assessed as transcriptional activation after 24 hrs by luciferase/beta-galactosidase reporter gene assay Agonist activity at human Gal4-fused ERalpha expressed in HEK293 cells assessed as transcriptional activation after 24 hrs by luciferase/beta-galactosidase reporter gene assay	[PMID: 31980362]
HEK293	IC₅₀	13 μM Compound: 16	Antagonist activity at human Gal4-fused ERalpha expressed in HEK293 cells assessed as inhibition of E2-induced transcriptional activation after 24 hrs by luciferase/beta-galactosidase reporter gene assay Antagonist activity at human Gal4-fused ERalpha expressed in HEK293 cells assessed as inhibition of E2-induced transcriptional activation after 24 hrs by luciferase/beta-galactosidase reporter gene assay	[PMID: 31980362]
HEK293	IC₅₀	5 μM Compound: 16	Antagonist activity at human Gal4-fused ERbeta expressed in HEK293 cells assessed as inhibition of E2-induced transcriptional activation after 24 hrs by luciferase/beta-galactosidase reporter gene assay Antagonist activity at human Gal4-fused ERbeta expressed in HEK293 cells assessed as inhibition of E2-induced transcriptional activation after 24 hrs by luciferase/beta-galactosidase reporter gene assay	[PMID: 31980362]
MCF7	EC₅₀	16 nM Compound: 16	Estrogenic activity in human MCF7 cells assessed as cell proliferation after 7 days by WST-8 assay Estrogenic activity in human MCF7 cells assessed as cell proliferation after 7 days by WST-8 assay	[PMID: 23214428]

Molecular Weight

228.36

Formula

C₁₄H₁₆OSi

CAS No.

74027-99-9

Appearance

Oil

Color

Colorless to light yellow

SMILES

OC1=CC=C([Si](C)(C)C2=CC=CC=C2)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (437.91 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.3791 mL	21.8953 mL	43.7905 mL
5 mM	0.8758 mL	4.3791 mL	8.7581 mL
10 mM	0.4379 mL	2.1895 mL	4.3791 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.87%

Select Batch:

Data Sheet (272 KB) SDS (251 KB)

COA (247 KB) HNMR (237 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Saito H, et al. Phosphine boranes as less hydrophobic building blocks than alkanes and silanes: Structure-property relationship and estrogen-receptor-modulating potency of 4-phosphinophenol derivatives. Bioorg Med Chem. 2020 Feb 15;28(4):115310. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.3791 mL	21.8953 mL	43.7905 mL	109.4763 mL
	5 mM	0.8758 mL	4.3791 mL	8.7581 mL	21.8953 mL
	10 mM	0.4379 mL	2.1895 mL	4.3791 mL	10.9476 mL
	15 mM	0.2919 mL	1.4597 mL	2.9194 mL	7.2984 mL
	20 mM	0.2190 mL	1.0948 mL	2.1895 mL	5.4738 mL
	25 mM	0.1752 mL	0.8758 mL	1.7516 mL	4.3791 mL
	30 mM	0.1460 mL	0.7298 mL	1.4597 mL	3.6492 mL
	40 mM	0.1095 mL	0.5474 mL	1.0948 mL	2.7369 mL
	50 mM	0.0876 mL	0.4379 mL	0.8758 mL	2.1895 mL
	60 mM	0.0730 mL	0.3649 mL	0.7298 mL	1.8246 mL
	80 mM	0.0547 mL	0.2737 mL	0.5474 mL	1.3685 mL
	100 mM	0.0438 mL	0.2190 mL	0.4379 mL	1.0948 mL