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  3. Fluorescent Dye
  4. Flubi-2

Flubi-2 is a fluorescein-biotin based pH-ratio dye (Ex=480 nm, Em= 520-560 nm) with a pK value of 6.7. Flubi-2 is a hydrolysis product of Flubida-2 (non-fluorescent, membrane permeable). Flubi-2 can be used for pH determination of organelles of the secretory pathway (such as golgi apparatus and endoplasmic reticulum) in living cells.

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Flubi-2 Chemical Structure

Flubi-2 Chemical Structure

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Based on 1 publication(s) in Google Scholar

Flubi-2 Related Antibodies

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Description

Flubi-2 is a fluorescein-biotin based pH-ratio dye (Ex=480 nm, Em= 520-560 nm) with a pK value of 6.7. Flubi-2 is a hydrolysis product of Flubida-2 (non-fluorescent, membrane permeable). Flubi-2 can be used for pH determination of organelles of the secretory pathway (such as golgi apparatus and endoplasmic reticulum) in living cells[1][2].

Molecular Weight

728.85

Formula

C78H88N8O16S2
Emission (Em)

517
Excitation (Ex)

492
SMILES

O=C1C=CC(C(OC2=CC(O)=CC=C23)=C1)=C3C4=CC=C(C(N(C)CCCCCCN(C)C(CCCC[C@@]5([H])C(NC(N6)=O)C6CS5)=O)=O)C=C4C(O)=O.O=C7C=CC(C(OC8=CC(O)=CC=C89)=C7)=C9C%10=CC(C(N(C)CCCCCCN(C)C(CCCC[C@@]%11([H])C(NC(N%12)=O)C%12CS%11)=O)=O)=CC=C%10C(O)=O
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Flubi-2 Related Classifications

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Flubi-2Flubi2Flubi 2Fluorescent DyeGolgipHendoplasmic reticulumFlubida-2Inhibitorinhibitorinhibit

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