Fluorescent Brightener 28  (Synonyms: Calcofluor White M2R)

Fluorescent brightener 28 is a fluorescent whitening agent commonly used in the padding process of the textile industry. Fluorescent brightener 28 is capable of staining polysaccharides such as cellulose, and when the plasma membrane ruptures, it also weakly stains the cytoplasm and strongly stains the cell nucleus. Additionally, Fluorescent brightener 28 can be utilized to detect intracellular chitin in living cells. Fluorescent Brightener 28 also is a visible light emitting diode (LED)-light sensitive photoinitiator for free radical photopolymerizations^[1][2][3].

960.95

C₄₀H₄₂N₁₂Na₂O₁₀S₂

4193-55-9

Liquid

Light yellow to yellow

O=S(C1=CC(NC2=NC(NC3=CC=CC=C3)=NC(N(CCO)CCO)=N2)=CC=C1/C=C/C4=CC=C(NC5=NC(NC6=CC=CC=C6)=NC(N(CCO)CCO)=N5)C=C4S(=O)(O[Na])=O)(O[Na])=O

Room temperature in continental US; may vary elsewhere.

Solution, -20°C, protect from light, 2 years

• [1]. Levene R, et al. The Enhancement of Fluorescence During Pad-Batch Whitening of Cotton Cloth 1[J]. Textile Research Journal, 1981, 51(9): 559-563.

  [2]. Nakazato Y,et al. Live Detection of Intracellular Chitin in Butterfly Wing Epithelial Cells In Vivo Using Fluorescent Brightener 28: Implications for the Development of Scales and Color Patterns. Insects. 2023 Sep 8;14(9):753.  [Content Brief]

  [3]. Xiaoling Zuo, et al. Fluorescent Brighteners as Visible LED-Light Sensitive Photoinitiators for Free Radical Photopolymerizations. Macromol Rapid Commun. 2016 May;37(10):840-4.  [Content Brief]