Fura Red AM 

Fura Red AM is a fluorescent probe that can be used to visualize [Ca(2+)] distribution in the cytoplasm of undifferentiated cells. Fura Red AM operates at an excitation wavelength of 488 nm^[1].

Fura Red AM is only used for undifferentiated cells, while differentiating cells exhibits strong autofluorescence in Fura Red channel. The stock liquor of Fura Red AM is recommended to be prepared in dimethyl sulfoxide with an original concentration of 45.91 mM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1088.99

C₄₇H₅₂N₄O₂₄S

218280-33-2

Solid

Orange to red

O=C(C)OCOC(CN(CC(OCOC(C)=O)=O)C1=CC=C(C)C=C1OCCOC2=CC3=C(C=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)OC(/C=C4C(N(COC(C)=O)C(N\4COC(C)=O)=S)=O)=C3)=O

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Walczysko P, et al. Use of co-loaded Fluo-3 and Fura Red fluorescent indicators for studying the cytosolic Ca(2+)concentrations distribution in living plant tissue. Cell Calcium. 2000 Jul;28(1):23-32.  [Content Brief]