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  3. Glutamate dehydrogenase

Glutamate dehydrogenase is an enzyme in both prokaryotes and eukaryotic mitochondria. Glutamate dehydrogenase can be used for the enzymatic determination of ammonia, alpha-ketoglutaric acid, L-glutamate and urease.

For research use only. We do not sell to patients.

Glutamate dehydrogenase Chemical Structure

Glutamate dehydrogenase Chemical Structure

CAS No. : 9001-46-1

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Description

Glutamate dehydrogenase is an enzyme in both prokaryotes and eukaryotic mitochondria. Glutamate dehydrogenase can be used for the enzymatic determination of ammonia, alpha-ketoglutaric acid, L-glutamate and urease[1].

In Vitro

Product Information
This product is recombinantly produced and can be used for the enzymatic determination of ammonia, α-ketoglutarate and L-glutamate, as well as the determination of leucine aminopeptidase and urease. In clinical analysis, this enzyme is combined with urease and can also be used for the enzymatic determination of urea.

Isoelectric point: 5.6
Michaelis constant: 9.21×10-3M (NH3), 4.80×10-3M(α-ketoglutarate), 7.8×10-5M (L-glutamate), 1.29×10-4M(NADH), 5.89×10-4M(NAD+)
Optimum pH: 7.0-9.0
Optimum temperature: 50-55℃
pH stability: 5.0-10.0 (25℃, 20hr)
Thermal stability: below 50℃ (pH 8.3, 10min)
Inhibitors: heavy metals, PCMB, IAA

Instructions
Soluble in 10 mM phosphate buffer (pH 7.0)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

[Glutamate dehydrogenase]

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Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL

*"≥" means soluble, but saturation unknown.

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Glutamate dehydrogenase
Cat. No.:
HY-E70003
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