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  4. HiBiT tag

HiBiT tag is a complimentary peptide (VSGWRLFKKIS), with high affinity for LgBiT. HiBiT tag is fused to the Gβ subunit of the receptor. The complementation of LgBiT and HiBiT facilitates the stabilization of ETR and G protein. HiBiT is attached to a protein of interest (GFP) to quantify transport to the cytosol.

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HiBiT tag Chemical Structure

HiBiT tag Chemical Structure

CAS No. : 1628912-54-8

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HiBiT tag Related Antibodies

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Description

HiBiT tag is a complimentary peptide (VSGWRLFKKIS), with high affinity for LgBiT. HiBiT tag is fused to the Gβ subunit of the receptor. The complementation of LgBiT and HiBiT facilitates the stabilization of ETR and G protein. HiBiT is attached to a protein of interest (GFP) to quantify transport to the cytosol[1][2].

Molecular Weight

1320.58

Formula

C63H101N17O14
CAS No.
Sequence

Val-Ser-Gly-Trp-Arg-Leu-Phe-Lys-Lys-Ile-Ser
Sequence Shortening

VSGWRLFKKIS
SMILES

O=C(N[C@@H](CO)C(NCC(N[C@@H](CC1=CNC2=CC=CC=C12)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CC(C)C)C(N[C@@H](CC3=CC=CC=C3)C(N[C@@H](CCCCN)C(N[C@@H](CCCCN)C(N[C@@H]([C@@H](C)CC)C(N[C@@H](CO)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)[C@H](C(C)C)N
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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HiBiT tag Related Classifications

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HiBiT tag1628912-54-8OthersInhibitorinhibitorinhibit

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