HXR9 hydrochloride

Name: HXR9 hydrochloride
Brand: MedChemExpress
SKU: HY-P3245A
Rating: 4.5 (1 reviews)

Cat. No.: HY-P3245A Purity: 99.20%: FAQs
Data Sheet SDS COA Handling Instructions

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HXR9 hydrochloride is a cell-permeable peptide and a competitive antagonist of HOX/PBX interaction. HXR9 hydrochloride antagonizes the interaction between HOX and a second transcrip-tion factor (PBX), which binds to HOX proteins in paralogue groups1 to 8. HXR9 hydrochloride selectively decreases cell proliferation and promotes apoptosis in cells with a high level of expression of the HOXA/PBX3 genes, such as MLL-rearranged leukemic cells.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

HXR9 hydrochloride Chemical Structure

Size	Price	Stock	Quantity
1 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 370	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 590	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of HXR9 hydrochloride:

HXR9 Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

HXR9 hydrochloride (60μM; 4 hours) blocks the interaction between PBX and HOX^[1].
HXR9 hydrochloride (60μM; 2 hours) triggers apoptosis in B16 and primary melanoma cells^[1].
HXR9 hydrochloride (60μM; 2 hours) causes specific transcriptional changes^[1].
HXR9 hydrochloride (B16 cells) shows antiproliferative activity with an IC₅₀ of 20μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line:	murine B16melanoma cells
Concentration:	60 μM
Incubation Time:	4 hours
Result:	Blocked the binding of HOXD9 to PBX.

Apoptosis Analysis

Cell Line:	B16 cells
Concentration:	60 μM
Incubation Time:	2 hours
Result:	A significant proportion of cells were in late phases of apoptosis.

RT-PCR

Cell Line:	B16F10cells
Concentration:	60 μM
Incubation Time:	2 hours
Result:	Fos, Jun, Dusp1, and Atf1，were allsignificantly up-regulate.

In Vivo

HXR9 hydrochloride (10 mg/kg; i.v. via the tail vein; twice weekly) blocks tumor growth^[1].
HXR9 hydrochloride (Initial dose of 100 mg/kg (subsequent dosing of 10 mg/kg twice weekly); Intraperitoneal; twice weekly for 18 days) blocks A549 tumour growth in vivo^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57black/6 mice (bearing B16 cells)
Dosage:	10 mg/kg
Administration:	I.v. via the tail vein; twice weekly (~30 days)
Result:	Tumors showed a significant degree of growth retardation.

Animal Model:	Athymic nude mice (bearing A549 cells)
Dosage:	Initial dose of 100 mg/kg (subsequent dosing of 10 mg/kg twice weekly)
Administration:	Intraperitoneal; twice weekly for 18 days
Result:	The tumours of HXR9-treated mice were considerably smaller than those of the control groups.

Molecular Weight

2754.67

Formula

C₁₁₉H₁₉₄ClN₅₃O₂₀S

Appearance

Solid

Color

White to off-white

Sequence Shortening

WYPWMKKHHRRRRRRRRR

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light, under nitrogen

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (18.15 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.3630 mL	1.8151 mL	3.6302 mL
5 mM	0.0726 mL	0.3630 mL	0.7260 mL
10 mM	0.0363 mL	0.1815 mL	0.3630 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (36.30 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.20%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (242 KB) RP-HPLC (202 KB) Elemental Analysis Report (101 KB)

Handling Instructions (2659 KB)

References

[1]. Morgan R, et al. Antagonism of HOX/PBX dimer formation blocks the in vivo proliferation of melanoma. Cancer Res. 2007;67(12):5806-5813. [Content Brief]

[2]. Li Z, et al. PBX3 is an important cofactor of HOXA9 in leukemogenesis. Blood. 2013;121(8):1422-1431. [Content Brief]

[3]. Plowright L, et al. HOX transcription factors are potential therapeutic targets in non-small-cell lung cancer (targeting HOX genes in lung cancer). Br J Cancer. 2009;100(3):470-475. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	0.3630 mL	1.8151 mL	3.6302 mL	9.0755 mL
	5 mM	0.0726 mL	0.3630 mL	0.7260 mL	1.8151 mL
	10 mM	0.0363 mL	0.1815 mL	0.3630 mL	0.9075 mL
	15 mM	0.0242 mL	0.1210 mL	0.2420 mL	0.6050 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.