High-Affinity Iodoacetyl Agarose

Cat. No.: HY-K0219: FAQs
Manual SDS

MCE High-Affinity Iodoacetyl Agarose, a 4% highly cross-linked agarose reagent coupled with a derivative of iodoacetic acid, is ideal for conjugating sulfhydryl-containing peptide or protein for subsequent affinity purification.

Size	Price	Stock	Quantity
Free Sample (500 μL)		Apply Now
5 mL	USD 180	In-stock	Estimated Time of Arrival: December 31
10 mL	USD 308	In-stock	Estimated Time of Arrival: December 31
50 mL	USD 1170	In-stock	Estimated Time of Arrival: December 31

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Description
Storage
Components
Documentation

Description
& Advantages

MCE High-Affinity Iodoacetyl Agarose, a 4% highly cross-linked agarose reagent coupled with a derivative of iodoacetic acid, reacts specifically with free sulfhydryls of sulfhydryl-containing peptides, proteins or other ligands and form stable thioether linkage. The agarose is ideal for conjugating sulfhydryl-containing peptide or protein for subsequent affinity purification. 1 mL of agarose can immobilize approximately 1 mg sulfhydryl-containing peptides of 7 amino-acids.

Characteristics
Composition	4% cross-linked agarose
Mean Bead Diameter	95 μm
pH Stability	pH 5-10
Binding Capacity	> 3 mg Goat IgG/mL settled resin
Storage Solution	1× PBS with 20% ethanol (pH 8.0)

The specifications of the product correspond to the actual resin volume, with the resin content of 50%.

Storage

Store at 2-8oC, and is stable for at least 2 years.

Do not dry or freeze.

Components

Components	HY-K0219-5 mL	HY-K0219-10 mL	HY-K0219-50 mL
High-Affinity Iodoacetyl Agarose (Settled Resin)	5 mL	10 mL	50 mL

Documentation

Manual (221 KB) SDS (115 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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