IP/Co-IP Kit (Protein A/G Magnetic Beads) 

MCE IP/Co-IP Kit (Protein A/G Magnetic Beads) is a kit developed based on Protein A/G magnetic beads. The kit includes recombinant Protein A/G magnetic beads and optimally validated reagents necessary for immunoprecipitation (IP) experiments. It can be used for protein purification, IP, Co-IP of target proteins or their protein complexes.

The kit contains sufficient reagents to perform 40 reactions using 25μL of magnetic beads.

Feature of MCE IP/Co-IP Kit (Protein A/G Magnetic Beads)

Compatible: Based on recombinant protein A/G magnetic beads, compatible with a wide range of antibody species.

Universal: Suitable for cell lysates, cell culture supernatants, serum, ascitic fluid, and other sample types.

Convenient: The kit includes magnetic beads and all necessary buffers required to complete the IP experiment.

Protein A/G Magnetic Beads: 4°C, 2 years. Do not freeze.

Elution Buffer, Neutralization Buffer: 4°C, 2 years.

IP Lysis Buffer, Protease Inhibitor Cocktail (100×), PMSF (100×),Protein Loading Buffer (Reducing, 4×), Protein Loading Buffer (Non-Reducing, 5×): -20°C, 2 years.

TBS-T Powder: RT, 2 years.

1. Do not centrifuge, dry, or freeze the magnetic beads. These operations may cause the beads to aggregate and reduce their binding capacity.

2. Before use, confirm the antibody subclass and its affinity with Protein A/G by referring to the appendix. If the affinity is poor, you can improve the binding efficiency by increasing the incubation time of the antibody with the magnetic beads (30 - 120 min), raising the pH of the binding buffer (8 - 9), and reducing the ionic strength (25 - 100 mM NaCl).

3. Ultrasonication will cause the antibody captured by the magnetic beads to detach from the beads. Therefore, after capturing the antibody, avoid using this method to resuspend the magnetic beads.

4. This product is for R&D use only, not for drug, household, or other uses.

5. For your safety and health, please wear a lab coat and disposable gloves to operate.

1. Do not centrifuge, dry, or freeze the magnetic beads. These operations may cause the beads to aggregate and reduce their binding capacity.

2. Before use, confirm the antibody subclass and its affinity with Protein A/G by referring to the appendix. If the affinity is poor, you can improve the binding efficiency by increasing the incubation time of the antibody with the magnetic beads (30 - 120 min), raising the pH of the binding buffer (8 - 9), and reducing the ionic strength (25 - 100 mM NaCl).

3. Ultrasonication will cause the antibody captured by the magnetic beads to detach from the beads. Therefore, after capturing the antibody, avoid using this method to resuspend the magnetic beads.

4. This product is for R&D use only, not for drug, household, or other uses.

5. For your safety and health, please wear a lab coat and disposable gloves to operate.