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  5. Lipid Concentrate (Chemically Defined)

Lipid Concentrate (Chemically Defined) 

Cat. No.: HY-K3019
Manual SDS

MCE Lipid Concentrate (Chemically Defined) is a concentrated lipid solution with a clearly defined chemical composition, which can be added to cell culture media as an additive for serum-free or low-serum cell culture systems.

Lipid Concentrate (Chemically Defined)
Size Price Stock
10 mL Ask For Quote & Lead Time
100 mL Ask For Quote & Lead Time

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  • Description

  • Storage

  • Application

  • Protocol

  • Attention

  • Components

  • Documentation

Description
& Advantages

MCE Lipid Concentrate (Chemically Defined) is a concentrated lipid solution with a clearly defined chemical composition, which can be added to cell culture media as an additive for serum-free or low-serum cell culture systems. It is suitable for the growth and maintenance of various cell types, such as CHO cells, hybridoma cells, and insect cells. It is widely used in applications such as protein expression in CHO cells, monoclonal antibody production by hybridomas, and virus expression (e.g., vaccines) and biopharmaceutical production in insect cells.

Storage

4℃, 1 year

Keep away from light, do not freeze.

Application

1. This product is an emulsion, appearing as a clear to milky white suspension liquid.

2. This product contains a surfactant and therefore may not be suited for growth of some adherent cell lines. Addition of this product to media containing a surfactant may result in cellular toxicity.

3. Minimize exposure to air. This product contains unsaturated fatty acids, which may oxidize when exposed to air. It is recommended to aliquot the product after opening and minimizes the headspace or fills it with inert gas.

4. Ensure aseptic techniques to avoid contamination. If contamination occurs during use, it is not recommended to filter sterilize, as this may remove the emulsion’s active components.

5. This product is for R&D use only, not for drug, household, or other uses.

6. For your safety and health, please wear a lab coat and disposable gloves to operate.

Protocol

According to experimental requirements, take an appropriate amount of Lipid Concentrate (Chemically Defined) in a sterile environment and add it directly to the cell culture medium. It is suitable for culturing cells in low-serum conditions.

Note: This product has been tested in a variety of cell lines grown in suspension culture at dilutions ranging from 1:100 to 1:1000.

Attention

1. This product is an emulsion, appearing as a clear to milky white suspension liquid.

2. This product contains a surfactant and therefore may not be suited for growth of some adherent cell lines. Addition of this product to media containing a surfactant may result in cellular toxicity.

3. Minimize exposure to air. This product contains unsaturated fatty acids, which may oxidize when exposed to air. It is recommended to aliquot the product after opening and minimizes the headspace or fills it with inert gas.

4. Ensure aseptic techniques to avoid contamination. If contamination occurs during use, it is not recommended to filter sterilize, as this may remove the emulsion’s active components.

5. This product is for R&D use only, not for drug, household, or other uses.

6. For your safety and health, please wear a lab coat and disposable gloves to operate.

Components
Components HY-K3019-10 mL HY-K3019-100 mL
Lipid Concentrate (Chemically Defined) 10 mL 100 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Lipid Concentrate (Chemically Defined)
Cat. No.:
HY-K3019
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