Basement Membrane Matrix for Organoid Culture 

MCE Basement Membrane Matrix for Organoid Culture is primarily composed of natural basement membrane matrix extracted from mouse tumors. This product is mainly used for organoid culture.

-20°C, 2 years.

Avoid repeated freezing and thawing.

For cell migration and invasion, primary cell expansion: Dilute with pre-cooled medium according to experimental needs after thawing. It is recommended that the concentration be no less than 1 mg/mL. Add 50 μL/cm² of matrix to the surface of the cell culture plate and spread it evenly, taking care to avoid air bubbles. Place the plate at 37 ℃ and incubate for 0.5 h, aspirate the supernatant to use.

For primary cell expansion: Dilute with pre-cooled medium according to experimental needs after thawing. It is recommended that the concentration be no less than 0.1 mg/mL. Aspirate the appropriate amount of dilution to completely cover the surface of the cell culture plate, the recommended amount of coating is 0.01-0.02 mg/cm². Place the plate at 37 ℃ and incubate for 1h, aspirate the supernatant to use.

For organoid culture and tumor spheroid invasion: Thaw the product in advance and set aside. Prepare the desired cells and resuspend them with matrix, it is recommended that the proportion of matrix is >70%. Add 15-20 μL/cm² to the surface of the cell culture plate, taking care to avoid air bubbles. Place the plate at 37 ℃ for 0.5 h to form a gel that wraps the cells, and add the appropriate medium to the plate.