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  5. Basement Membrane Matrix
  6. Basement Membrane Matrix IPSC-qualified

Basement Membrane Matrix IPSC-qualified 

Cat. No.: HY-K6006
Manual SDS

MCE Basement Membrane Matrix IPSC-qualified is primarily composed of natural basement membrane matrix extracted from mouse tumors. This product is mainly used for stem cells culture.

Basement Membrane Matrix IPSC-qualified
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5 mL USD 239 In-stock
10 mL USD 435 In-stock
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MCE Biological Validation

Stem Cell Culture
Differentiation Induction
    Basement Membrane Matrix IPSC-qualified (HY-K6006) (0.015 mg/cm2) is capable of maintaining the culture of stem cell (WTC and H9 cells) without differentiation.
    HY-K6006 induces the differentiation of iPSCs into cardiomyocytes.

    • Description

    • Storage

    • Protocol

    • Components

    • Documentation

    Description
    & Advantages

    MCE Basement Membrane Matrix IPSC-qualified is primarily composed of natural basement membrane matrix extracted from mouse tumors. This product is mainly used for stem cells culture.
    Storage

    -20°C, 2 years.

    Avoid repeated freezing and thawing.
    Protocol
    Thin Coating Method.

    For iPSC expansion: Dilute with pre-cooled medium according to experimental needs after thawing. It is recommended that the concentration be no less than 0.1 mg/mL. Aspirate the appropriate amount of dilution to completely cover the surface of the cell culture plate, the recommended amount of coating is 0.01-0.02 mg/cm2. Place the plate at 37 ℃ and incubate for 1h, aspirate the supernatant to use.

    Sandwich Coating Method.

    For stem cell differentiation: After Thin Coating Method, when the cells are grown to a suitable state, the supernatant is aspirated off and matrix dilution is added to the cell surface to completely cover the surface of the cell culture plate and shaken well. Incubate the plate at 37 ℃ for 1h, aspirate off the supernatant, and add the appropriate medium to the plate.

     

    Note: When thawing the product for the first time, immerse the vial in crushed ice and place it in a 4°C to melt. After melting, mix thoroughly before aliquoting, and then store at -20°C.
    Components
    Components HY-K6006-5 mL HY-K6006-10 mL
    Basement Membrane Matrix IPSC-qualified 5 mL 5 mL ×2
    Documentation
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Basement Membrane Matrix IPSC-qualified
    Cat. No.:
    HY-K6006
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