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  5. Basement Membrane Matrix
  6. Basement Membrane Matrix (Phenol Red)

Basement Membrane Matrix (Phenol Red) 

Cat. No.: HY-K6001
Manual SDS

MCE Basement Membrane Matrix (Phenol Red) is primarily composed of a natural basement membrane matrix derived from mouse tumors. This product is mainly for studies of tumor invasion, angiogenesis, and organoid culture.

Basement Membrane Matrix (Phenol Red)
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5 mL USD 182 In-stock
10 mL USD 343 In-stock
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    Basement Membrane Matrix (Phenol Red) purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 May 1;10(9):e30603.  [Abstract]

    After overexpression of RBMS3 and activation of the Wnt/β-catenin signalling pathway, the migratory activity of ID8 cells was detected by transwell.

    Basement Membrane Matrix (Phenol Red) purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 May 1;10(9):e30603.  [Abstract]

    After overexpression of RBMS3 and activation of the Wnt/β-catenin signalling pathway, the invasive activity of ID8 cells was detected by transwell.

    Basement Membrane Matrix (Phenol Red) purchased from MedChemExpress. Usage Cited in: ACS Omega. 2024 Jan 16;9(4):4974-4985.

    Transwell results after HUs culturing with different Cu2O nanoparticle concentrations.

    Basement Membrane Matrix (Phenol Red) purchased from MedChemExpress. Usage Cited in: ACS Omega. 2024 Jan 16;9(4):4974-4985.

    Angiogenesis images after HUs culturing with different Cu2O nanoparticle concentrations.

    MCE Biological Validation

    Transwell assays
    Angiogenesis Assay
      5×104 SGC-7901 cells are cultured for 44 hours under different concentrations of Basement Membrane Matrix (Phenol Red) (HY-K6001) (HY-K6001 : basal medium=0:1; 1:8; 1:6; 1:4), and the cell invasion rate decreases with the increase of Matrix concentration.
      HUVEC cells are cultured for 4 hours under Basement Membrane Matrix (Phenol Red) (HY-K6001) (HY-K6001 : Basal Medium = 1:1), and displays tubular structure formation.
      U87 cells are cultured for 4 hours under Basement Membrane Matrix (Phenol Red) (HY-K6001) (HY-K6001 : Basal Medium = 1:1), and displays tubular structure formation.

      • Description

      • Storage

      • Protocol

      • Components

      • Documentation

      Description
      & Advantages

      MCE Basement Membrane Matrix (Phenol Red) is a natural basement membrane matrix extracted from mouse tumors and is composed mainly of various growth factors and extracellular matrix components. The main extracellular matrix components are: Laminin, Col-IV, Entactin, Heparan sulphate proteoglycans, etc. The main growth factor components are: insulin-like growth factor (IGF-1), transforming growth factor beta (TGF-β), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), fibroblast growth factor (bFGF), etc.

      MCE Basement Membrane Matrix (Phenol Red) is suitable for studies of tumor invasion, angiogenesis, and organoid culture.
      Storage

      -20°C, 2 years.

      Avoid repeated freezing and thawing.
      Protocol

      a. Use a pre-cooled pipette to blow the matrix gel evenly after thawing.

      b. Use pre-cooled serum-free medium to dilute according to experimental requirements. Refer to the Experimental Conditions table for final concentrations.

      c. Place the culture plate on ice and inject the substrate gel dilution slowly against the wall to avoid air bubbles. Refer to the Experimental Conditions table for the volume injected.

      d. Transfer the culture plate to 37℃ incubator gently. After gelling, aspirate off excess supernatant. Refer to the Experimental Conditions table for gel time.

       

      Note: When thawing the product for the first time, immerse the vial in crushed ice and place it in a 4°C to melt. After melting, mix thoroughly before aliquoting, and then store at -20°C.
      Components
      Components HY-K6001-5 mL HY-K6001-10 mL
      Basement Membrane Matrix (Phenol Red) 5 mL 10 mL
      Documentation
      Help & FAQs
      • Do most proteins show cross-species activity?

        Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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      Product Name:
      Basement Membrane Matrix (Phenol Red)
      Cat. No.:
      HY-K6001
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