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  5. Protease Inhibitor Cocktail
  6. Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH2O)

Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH2O) 

Cat. No.: HY-K0013
Manual SDS

MCE Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH2O) protects protein from degradation by endogenous proteases released during protein extraction and purification.

Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH<sub>2</sub>O)
Size Price Stock Quantity
Free Sample (100 μL)   Apply Now  
1 mL USD 50 In-stock
10 mL USD 350 In-stock
50 mL USD 1380 In-stock
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85 Publications Citing Use of MCE Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH2O)

WB

    Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH2O) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 May 8:e2305925.  [Abstract]

    Temporal dynamics of the ratio between Cry1 and housekeeping gene Actin expression levels following SS treatment in western blot analysis. Each dot stands for the relative protein expression of Cry1, which were fitted to the cosine function, and the p‐value was calculated.

    • Description

    • Storage

    • Protocol

    • Components

    • Documentation

    Description
    & Advantages

    MCE Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH2O) protects protein from degradation by endogenous proteases released during protein extraction and purification.

    This cocktail is AEBSF free and mass spectroscopy compatible�Cwater soluble. The protease inhibitors target serine proteases, aminopeptidase B, leucine aminopeptidase, cysteine proteases, metalloproteases. The phosphatase inhibitors target serine/threonine phosphatases, acid phosphatases, protein tyrosine.

    Storage

    -20°C, 2 years
    Protocol

    1.    This product can be applied in Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.

    2.    Thaw at room temperature, add at 1:10 (v/v) dilution to 1 solution samples, mix throughly. It is recommended to prepare solutions of this product fresh when needed.

    3.    If necessary, 0.5 mM EDTA can be prepared and added to the mix by the ratio of 1:10 (v/v).
    Components
    Cat. No. Ingredient Target Package
    HY-K0013 Aprotinin Serine proteases 1 mL
    10 mL (1 mL × 10)
    50 mL (1 mL × 50)
    Bestatin Aminopeptidases
    E-64 Cysteine proteases
    Leupeptin hemisulfate Serine and Cysteine proteases
    β-Glycerophosphate Serine and Threonine phosphatases
    Sodium fluoride Serine and Threonine phosphatases
    Sodium orthovanadate Tyrosine phosphatases
    Sodium pyrophosphate decahydrate Serine and Threonine phosphatases
    Documentation
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH2O)
    Cat. No.:
    HY-K0013
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