2. MCE Kits
  3. Protein Biology
  4. Protein Purification
  5. Agarose Beads
  6. Protein G Agarose

Protein G Agarose 

Cat. No.: HY-K0214
Manual SDS

MCE Protein G Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein G, effectively purifies mammalian monoclonal and polyclonal antibodies, such as human IgG3 and rat IgG2a.

Protein G Agarose
Size Price Stock Quantity
Free Sample (500 μL)   Apply Now  
5 mL USD 230 In-stock
10 mL USD 380 In-stock
50 mL USD 1735 In-stock
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2 Publications Citing Use of MCE Protein G Agarose

  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

Protein G is a bacterial cell wall protein isolated from group G Streptococci and binds to most mammalian IgGs mainly through Fc regions. Native Protein G contains 3 IgG binding domains and sites for albumin and cell surface binding. The latter have been eliminated from recombinant Protein G to reduce nonspecific binding.


MCE Protein G Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein G, effectively purifies mammalian monoclonal and polyclonal antibodies, such as human IgG3 and rat IgG2a.


Characteristics
Composition 4% cross-linked agarose
Degree of Substitution 2 mg Recombinant Protein G/mL Settled Resin
Bead Diameter 45-165 μm
Binding Capacity 20 mg human IgG/mL Settled Resin
Storage Solution 50% slurry in 1× PBS containing 20% ethanol

 

The specifications of the product correspond to the actual resin volume, with the resin content of 50%.
Storage

Store at 2-8°C, and is stable for at least 2 years.


Do not dry or freeze.
Components
Components HY-K0214-5 mL HY-K0214-10 mL HY-K0214-50 mL
Protein G Agarose (Settled Resin) 5 mL 10 mL 50 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Protein G Agarose
Cat. No.:
HY-K0214
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