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  3. Janelia Fluor® 646, SE

Janelia Fluor® 646, SE  (Synonyms: JF646, SE; JF646, NHS)

Cat. No.: HY-130735 Purity: 96.39%
Handling Instructions Technical Support

Janelia Fluor 646, SE (JF646, SE) is a red fluorescent dye can be used in cellular imaging. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

For research use only. We do not sell to patients.

Janelia Fluor® 646, SE Chemical Structure

Janelia Fluor® 646, SE Chemical Structure

CAS No. : 1811539-59-9

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Janelia Fluor® 646, SE:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Janelia Fluor 646, SE (JF646, SE) is a red fluorescent dye can be used in cellular imaging[1]. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

In Vitro

Excitation maximum=646 nm; emission maximum=664 nm[1].
JF646 can serve as ligand for self-labeling tag (such as HaloTag)[1].
Janelia Fluor 646, SE (JF646, SE) is modified by JF646. JF646, a red fluorescent dye, is photostable, membrane-permeable, has a high extinction coefficient[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

593.70

Formula

C33H31N3O6Si

CAS No.
Appearance

Solid

Color

Light green to green

Emission (Em)

646

Excitation (Ex)

606

SMILES

C[Si]1(C)C2=C(C=CC(N3CCC3)=C2)C(C4=CC(C(ON5C(CCC5=O)=O)=O)=CC=C4C([O-])=O)=C6C=C/C(C=C16)=[N+]7CCC\7

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 12.5 mg/mL (21.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6844 mL 8.4218 mL 16.8435 mL
5 mM 0.3369 mL 1.6844 mL 3.3687 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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g

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 96.42%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6844 mL 8.4218 mL 16.8435 mL 42.1088 mL
5 mM 0.3369 mL 1.6844 mL 3.3687 mL 8.4218 mL
10 mM 0.1684 mL 0.8422 mL 1.6844 mL 4.2109 mL
15 mM 0.1123 mL 0.5615 mL 1.1229 mL 2.8073 mL
20 mM 0.0842 mL 0.4211 mL 0.8422 mL 2.1054 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Janelia Fluor® 646, SE
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