K1586

Cat. No.: HY-161622: FAQs
Data Sheet Handling Instructions

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K1586 is an amidine derivative that efficiently targets Chk1. K1586 enhances the degradation of Chk1 that sensitizes colorectal cancer cells to ionizing radiation. K1586 shows anticancer effects.

For research use only. We do not sell to patients.

K1586 Chemical Structure

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Chk1 Chk2

Biological Activity
Purity & Documentation
References
Customer Review

Description

K1586 is an amidine derivative that efficiently targets Chk1. K1586 enhances the degradation of Chk1 that sensitizes colorectal cancer cells to ionizing radiation. K1586 shows anticancer effects^[1].

In Vitro

K1586 (0.001-100 μM; 72 h) decreases cell proliferation in a dose-dependent manner^[1].
K1586 (10-20 μM; 24 h) increases the relative length of the S phase and significant increases the length of the G2/M phase^[1].
K1586 (10 μM; 12 h) decreases Chk1 protein levels in a time-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	HT29 human colorectal cancer cells
Concentration:	0.001 μM, 0.01 μM, 0.1 μM, 1 μM, 5 μM, 10 μM, 50 μM, and 100 μM
Incubation Time:	72 h
Result:	Proliferation decreased in a dose-dependent manner.

Cell Cycle Analysis^[1]

Cell Line:	HT29 cells
Concentration:	10 or 20 μM
Incubation Time:	24 h
Result:	Increased the length of the G2/M phase in cells.

Western Blot Analysis^[1]

Cell Line:	HT29 cells
Concentration:	10 μM
Incubation Time:	12 h
Result:	Induced Chk1 destabilization and degradation.

Molecular Weight

359.87

Formula

C₁₈H₁₈ClN₃OS

SMILES

CCN(CC)/C=N/C(C=C1)=CC2=C1N=C(S2)OC3=CC=C(Cl)C=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Hang Soo Kim, et al. Novel Amidine Derivative K1586 Sensitizes Colorectal Cancer Cells to Ionizing Radiation by Inducing Chk1 Instability. Int J Mol Sci. 2024 Apr 16;25(8):4396. [Content Brief]

K1586 Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

K1586K 1586K-1586Checkpoint Kinase (Chk)anticancer agentChk1 inhibitorionizing radiationamidine derivativeInhibitorinhibitorinhibit

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