2. Metabolic Enzyme/Protease Anti-infection
  3. Ser/Thr Protease Virus Protease Cathepsin
  4. Leupeptin

Leupeptin is a broad-spectrum, membrane-permeable protease inhibitor. Leupeptin potently inhibits serine, cysteine and threonine proteases. Leupeptin inhibits Mpro (the main protease of SARS-CoV-2) and also has anti-inflammatory activity.

For research use only. We do not sell to patients.

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Leupeptin Chemical Structure

Leupeptin Chemical Structure

CAS No. : 55123-66-5

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Leupeptin Related Antibodies

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Cathepsin B Cathepsin C Cathepsin D Cathepsin E Cathepsin K Cathepsin L Cathepsin S

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Description

Leupeptin is a broad-spectrum, membrane-permeable protease inhibitor. Leupeptin potently inhibits serine, cysteine and threonine proteases. Leupeptin inhibits Mpro (the main protease of SARS-CoV-2) and also has anti-inflammatory activity[1][2][3].

IC50 & Target

Cathepsin B, Cathepsin H, Cathepsin L, Ser/Thr Protease, Mpro[1][2][3].
In Vitro

Leupeptin (0.06-200 µM; 72 h) significantly decreases copy numbers of SARS-CoV-2 viral RNA (vRNA) in Vero cells[1]
Leupeptin inhibits RNA levels of SARS-CoV-2 in Vero cells, with an EC50 value of 42.34 µM[1].
Leupeptin has some inhibitory activity against Mpro, with an IC50 value of 127.2 µM[1].
Leupeptin againsts human coronavirus strain 229E with an IC50 value of 0.4 µg/mL (about 1 µM)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Leupeptin Related Antibodies

RT-PCR[1]

Cell Line: Vero cells
Concentration: 0.06-200 µM
Incubation Time: 72 h
Result: Significantly decreased copy numbers of SARS-CoV-2 viral RNA (vRNA).
In Vivo

Leupeptin (0, 9, 18, 36 mg/kg; i.p.; single) is well tolerated by the animals and produces a strong, dose-dependent increase in LC3b-II in both the total tissue extracts and the lysosome and autophagosome-enriched pellet fraction[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6NCrl male mice (6-8 weeks old, 20-25 g)[1].
Dosage: 0, 9, 18, 36 mg/kg
Administration: Intraperitoneal injection; single
Result: Promoted the accumulation of LC3b-II in mouse liver.
Molecular Weight

426.55

Formula

C20H38N6O4
CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Leupeptin Related Classifications

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Leupeptin55123-66-5Ser/Thr ProteaseVirus ProteaseCathepsinSerine proteasesSerine endopeptidasesThreonine proteasesMproSARS-CoV-2Vero cellscathepsinserine proteasesthreonine proteasescysteine proteasesInhibitorinhibitorinhibit

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