2. Academic Validation
  3. Phenylalkyl backbone modified oligodeoxynucleotides, their synthesis and the influence of the alkyl chain length

Phenylalkyl backbone modified oligodeoxynucleotides, their synthesis and the influence of the alkyl chain length

  • Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):1275-8. doi: 10.1081/NCN-100002535.
S Amberg 1,  J W Engels
Affiliations

Affiliation

  • 1 Institut für Organische Chemie, Johann Wolfgang Goethe-Universität, Marie Curie Strasse 11, D-60439 Frankfurt am Main, Germany.
PMID: 11563002 DOI: 10.1081/NCN-100002535
Abstract

Phenylalkyl modified phosphoramidites (alkyl chain length n = 1, 2, 3, 5; Fig. 1) were synthesised and incorporated into a DNA hexamer (5'-d(GCCp-GCG); p = place of modification). The obtained diastereomeres were separated by RP-HPLC. After hybridisation with the complementary DNA strand Tm-value and thermodynamic data were measured. The stability of duplexes depends on the linker length and the absolute configuration of the backbone modified oligodeoxynucleotides (Rp, Sp).

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