2. Academic Validation
  3. GHRH and IL1beta increase cytoplasmic Ca(2+) levels in cultured hypothalamic GABAergic neurons

GHRH and IL1beta increase cytoplasmic Ca(2+) levels in cultured hypothalamic GABAergic neurons

  • Brain Res. 2002 Sep 13;949(1-2):209-12. doi: 10.1016/s0006-8993(02)03157-8.
Alok De 1,  Lynn Churchill,  Ferenc Obal Jr,  Steven M Simasko,  James M Krueger
Affiliations

Affiliation

  • 1 Department of VCAPP, Program in Neuroscience, Washington State University, PO Box 646520, Pullman, WA 99164-6520, USA.
PMID: 12213318 DOI: 10.1016/s0006-8993(02)03157-8
Abstract

GHRH and IL1beta regulate sleep via the hypothalamus. However, actions of these substances on neurons are poorly understood. In this study, we found both GHRH (100 nM) and IL1beta (1.2 pM) acutely increased cytosolic CA(2+) in 7.6 and 4.0% of cultured hypothalamic neurons tested, respectively, and 1.2% of neurons responded to both. The neurons that responded were mostly GABAergic (96, 81, and 100% for GHRH, IL1beta, and dual-responsive neurons, respectively).

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