1. Academic Validation
  2. Identification of major metabolites in Aloe littoralis by high-performance liquid chromatography-nuclear magnetic resonance spectroscopy

Identification of major metabolites in Aloe littoralis by high-performance liquid chromatography-nuclear magnetic resonance spectroscopy

  • Phytochem Anal. 2003 Sep-Oct;14(5):275-80. doi: 10.1002/pca.714.
Gloria Karagianis 1 Alvaro Viljoen Peter G Waterman
Affiliations

Affiliation

  • 1 Centre for Phytochemistry, Southern Cross University, PO Box 157, Lismore, NSW 2480, Australia. gkaragia@scu.edu.au
Abstract

Examination of the leaf exudate of the South African species Aloe littoralis by reversed-phase HPLC revealed the presence of two major metabolites. The identification of the two compounds without isolation was attempted by HPLC-NMR based on separation using a C18 column eluting with a deuterium oxide:acetonitrile solvent gradient and an inverse HPLC-NMR probe. For each compound, one-dimensional proton spectra, and two-dimensional homonuclear COSY and TOCSY, and heteronuclear HSQC and HMBC, spectra were collected. On the basis of the data obtained, the metabolites were characterised as 10-hydroxyaloin B and deacetyllittoraloin.

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