A mechanism for the regulation of pre-mRNA 3' processing by human cleavage factor Im

Mol Cell. 2003 Dec;12(6):1467-76. doi: 10.1016/s1097-2765(03)00453-2.

Kirk M Brown ¹, Gregory M Gilmartin

Affiliations

Affiliation

1 Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT 05405, USA.

PMID: 14690600 DOI: 10.1016/s1097-2765(03)00453-2

Abstract

Human cleavage factor I(m) (CFI(m)) is a heterodimeric RNA binding protein complex that functions at an early step in the assembly of the pre-mRNA 3' processing complex. In this report we show that CFI(m) can stimulate both cleavage and poly(A) addition, and can act to suppress poly(A) site cleavage in a sequence-dependent manner. Elevated levels of CFI(m) suppressed cleavage at the primary poly(A) site of the pre-mRNA encoding the 68 kDa subunit of CFI(m). CFI(m)-mediated suppression of poly(A) site cleavage was dependent upon the presence of three copies of an RNA element initially identified by CFI(m)-SELEX. These data provide evidence for a mechanism for the regulation of poly(A) site selection by a basal pre-mRNA 3' processing factor.

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