High-throughput mapping of a dynamic signaling network in mammalian cells

Science. 2005 Mar 11;307(5715):1621-5. doi: 10.1126/science.1105776.

Miriam Barrios-Rodiles ¹, Kevin R Brown, Barish Ozdamar, Rohit Bose, Zhong Liu, Robert S Donovan, Fukiko Shinjo, Yongmei Liu, Joanna Dembowy, Ian W Taylor, Valbona Luga, Natasa Przulj, Mark Robinson, Harukazu Suzuki, Yoshihide Hayashizaki, Igor Jurisica, Jeffrey L Wrana

Affiliations

Affiliation

1 Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada, M5G 1X5.

PMID: 15761153 DOI: 10.1126/science.1105776

Abstract

Signaling pathways transmit information through protein interaction networks that are dynamically regulated by complex extracellular cues. We developed LUMIER (for luminescence-based mammalian interactome mapping), an automated high-throughput technology, to map protein-protein interaction networks systematically in mammalian cells and applied it to the transforming growth factor-beta (TGFbeta) pathway. Analysis using self-organizing maps and k-means clustering identified links of the TGFbeta pathway to the p21-activated kinase (PAK) network, to the polarity complex, and to Occludin, a structural component of tight junctions. We show that Occludin regulates TGFbeta type I receptor localization for efficient TGFbeta-dependent dissolution of tight junctions during epithelial-to-mesenchymal transitions.

Name
Email *

	Sorry, but the email address you supplied was invalid.