2. Academic Validation
  3. The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling

The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling

  • FEBS Lett. 2005 Aug 15;579(20):4317-23. doi: 10.1016/j.febslet.2005.06.061.
Wei He 1,  Ke Gong,  David K Smith,  Nancy Y Ip
Affiliations

Affiliation

  • 1 Department of Biochemistry and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.
PMID: 16051226 DOI: 10.1016/j.febslet.2005.06.061
Abstract

Ciliary neurotrophic factor (CNTF) forms a functional receptor complex containing the CNTF receptor, gp130, and the Leukemia Inhibitory Factor receptor (LIFR). However, the nature and stoichiometry of the receptor-mediated interactions in this complex have not yet been fully resolved. We show here that signaling by CNTF, but not by LIF or oncostatin M (OSM), was abolished in cells overexpressing a LIFR mutant with the N-terminal cytokine binding domain deleted. Our results illustrate molecular differences between the CNTF active receptor complex and those of LIF and OSM and provide further support for the hexameric model of the CNTF receptor complex.

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