2. Academic Validation
  3. Inducible nitric oxide synthase binds, S-nitrosylates, and activates cyclooxygenase-2

Inducible nitric oxide synthase binds, S-nitrosylates, and activates cyclooxygenase-2

  • Science. 2005 Dec 23;310(5756):1966-70. doi: 10.1126/science.1119407.
Sangwon F Kim 1,  Daniel A Huri,  Solomon H Snyder
Affiliations

Affiliation

  • 1 Department of Neuroscience, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA.
PMID: 16373578 DOI: 10.1126/science.1119407
Abstract

Cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) are two major inflammatory mediators. Here we show that iNOS specifically binds to COX-2 and S-nitrosylates it, enhancing COX-2 catalytic activity. Selectively disrupting iNOS-COX-2 binding prevented NO-mediated activation of COX-2. This synergistic molecular interaction between two inflammatory systems may inform the development of anti-inflammatory drugs.

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