Molecular anatomy of a trafficking organelle

Cell. 2006 Nov 17;127(4):831-46. doi: 10.1016/j.cell.2006.10.030.

Shigeo Takamori ¹, Matthew Holt, Katinka Stenius, Edward A Lemke, Mads Grønborg, Dietmar Riedel, Henning Urlaub, Stephan Schenck, Britta Brügger, Philippe Ringler, Shirley A Müller, Burkhard Rammner, Frauke Gräter, Jochen S Hub, Bert L De Groot, Gottfried Mieskes, Yoshinori Moriyama, Jürgen Klingauf, Helmut Grubmüller, John Heuser, Felix Wieland, Reinhard Jahn

Affiliations

Affiliation

1 Department of Neurobiology, Max-Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany.

PMID: 17110340 DOI: 10.1016/j.cell.2006.10.030

Abstract

Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor compartment and fuse with an acceptor compartment. Common principles of budding and fusion have emerged, and many of the proteins involved in these events are now known. However, a detailed picture of an entire trafficking organelle is not yet available. Using synaptic vesicles as a model, we have now determined the protein and lipid composition; measured vesicle size, density, and mass; calculated the average protein and lipid mass per vesicle; and determined the copy number of more than a dozen major constituents. A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins. Synaptic vesicles are dominated by proteins, possess a surprising diversity of trafficking proteins, and, with the exception of the V-ATPase that is present in only one to two copies, contain numerous copies of proteins essential for membrane traffic and neurotransmitter uptake.

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