1. Academic Validation
  2. Hexadecanedionic acid-sepharose 4B: A new tool for preparation of albumin-depleted plasma

Hexadecanedionic acid-sepharose 4B: A new tool for preparation of albumin-depleted plasma

  • J Proteome Res. 2006 Dec;5(12):3453-8. doi: 10.1021/pr060387q.
Vukic Soskic 1 Gerhard Schwall Elke Nyakatura Slobodan Poznanovic Werner Stegmann Andre Schrattenholz
Affiliations

Affiliation

  • 1 ProteoSys AG, Carl-Zeiss-Strasse 51, 55129 Mainz, Germany. vukic.soskic@proteosys.com
Abstract

Serum and plasma are the major sources of human material for clinical molecular diagnostics and drug discovery. However, due to the high abundance of some proteins, of which serum albumin (SA) is most prominent, lower-abundance proteins often remain undetectable in proteomic analysis of these body fluids. We have used hexadecanedionic acid (HDA) immobilized to Sepharose 4B to develop an affinity resin that is effective in the removal of SA from plasma. Two-dimensional gel analysis of the SA-depleted samples shows a significant enhancement of the low-abundance proteins and highly specific capture of serum albumin. The HDA resin shows better performance in terms of specificity than dye-based resins.

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