2. Academic Validation
  3. Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme

Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme

  • Mol Cell. 2007 Jul 20;27(2):262-274. doi: 10.1016/j.molcel.2007.06.027.
Célia Jeronimo 1,  Diane Forget 1,  Annie Bouchard 1,  Qintong Li 2,  Gordon Chua 3,  Christian Poitras 1,  Cynthia Thérien 1,  Dominique Bergeron 1,  Sylvie Bourassa 4,  Jack Greenblatt 3,  Benoit Chabot 5,  Guy G Poirier 4,  Timothy R Hughes 3,  Mathieu Blanchette 6,  David H Price 2,  Benoit Coulombe 7
Affiliations

Affiliations

  • 1 Laboratory of Gene Transcription and Proteomics Discovery Platform, Institut de recherches cliniques de Montréal, 110 avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada.
  • 2 Biochemistry Department, University of Iowa, Iowa City, IA 52242-1109, USA.
  • 3 Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5G 1L6, Canada.
  • 4 Centre hospitalier universitaire de Québec, Université Laval, Québec, QC G1V 4G2, Canada.
  • 5 Département de microbiologie et infectiologie, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada.
  • 6 McGill Centre for Bioinformatics, McGill University, Montréal, QC H3A 2B4, Canada.
  • 7 Laboratory of Gene Transcription and Proteomics Discovery Platform, Institut de recherches cliniques de Montréal, 110 avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada. Electronic address: benoit.coulombe@ircm.qc.ca.
PMID: 17643375 DOI: 10.1016/j.molcel.2007.06.027
Abstract

We have performed a survey of soluble human protein complexes containing components of the transcription and RNA processing machineries using protein affinity purification coupled to mass spectrometry. Thirty-two tagged polypeptides yielded a network of 805 high-confidence interactions. Remarkably, the network is significantly enriched in proteins that regulate the formation of protein complexes, including a number of previously uncharacterized proteins for which we have inferred functions. The RNA polymerase II (RNAP II)-associated proteins (RPAPs) are physically and functionally associated with RNAP II, forming an interface between the Enzyme and chaperone/scaffolding proteins. BCDIN3 is the 7SK snRNA methylphosphate capping Enzyme (MePCE) present in an snRNP complex containing both RNA processing and transcription factors, including the elongation factor P-TEFb. Our results define a high-density protein interaction network for the mammalian transcription machinery and uncover multiple regulatory factors that target the transcription machinery.

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