2. Academic Validation
  3. A pathway sensor for genome-wide screens of intracellular proteolytic cleavage

A pathway sensor for genome-wide screens of intracellular proteolytic cleavage

  • Genome Biol. 2008 Apr 3;9(4):R64. doi: 10.1186/gb-2008-9-4-r64.
Robin Ketteler 1,  Zairen Sun,  Karl F Kovacs,  Wei-Wu He,  Brian Seed
Affiliations

Affiliation

  • 1 Center for Computational and Integrative Biology, Massachusetts General Hospital, Cambridge Street, Boston, MA 02114, USA.
PMID: 18387192 DOI: 10.1186/gb-2008-9-4-r64
Abstract

Protein cleavage is a central event in many regulated biological processes. We describe a system for detecting intracellular proteolysis based on non-conventional secretion of Gaussia luciferase (GLUC). GLUC exits the cell without benefit of a secretory leader peptide, but can be anchored in the cell by fusion to beta-actin. By including Protease cleavage sites between GLUC and beta-actin, proteolytic cleavage can be detected. Using this assay, we have identified regulators of Autophagy, Apoptosis and beta-actin cleavage.

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