2. Academic Validation
  3. High-affinity NGF binding requires coexpression of the trk proto-oncogene and the low-affinity NGF receptor

High-affinity NGF binding requires coexpression of the trk proto-oncogene and the low-affinity NGF receptor

  • Nature. 1991 Apr 25;350(6320):678-83. doi: 10.1038/350678a0.
B L Hempstead 1,  D Martin-Zanca,  D R Kaplan,  L F Parada,  M V Chao
Affiliations

Affiliation

  • 1 Department of Medicine, Cornell University Medical College, New York, New York 10021.
PMID: 1850821 DOI: 10.1038/350678a0
Abstract

Nerve growth factor (NGF) interacts with two different low-affinity receptors that can be distinguished by affinity crosslinking. Reconstitution experiments by membrane fusion and transient transfection into heterologous cells indicate that high-affinity NGF binding requires coexpression and binding to both the low-affinity NGF receptor and the tyrosine kinase Trk gene product. These studies reveal a new growth factor receptor-mediated mechanism of cellular differentiation involving Trk and the low-affinity NGF receptor.

Figures