2. Academic Validation
  3. Synthesis and characterization of BODIPY-labeled colchicine

Synthesis and characterization of BODIPY-labeled colchicine

  • Bioorg Med Chem Lett. 2008 Nov 15;18(22):5867-70. doi: 10.1016/j.bmcl.2008.07.068.
Leggy A Arnold 1,  Patricia Ranaivo,  R Kiplin Guy
Affiliations

Affiliation

  • 1 St. Jude Children's Research Hospital, Department of Chemical Biology and Therapeutics, 332 N. Lauderdale St., Mail Stop 1000, Memphis, TN 38105-2794, USA.
PMID: 18684625 DOI: 10.1016/j.bmcl.2008.07.068
Abstract

Two BODIPY-labeled colchicine derivatives were synthesized and shown to bind to tubulin but only partially inhibit tubulin polymerization in the presence of GTP. Cytotoxicity studies were carried out in HeLa, HepG2, Raji and Vero cells. Apoptosis-inducing properties were determined by Caspase 3/7 activity and flow cytometry and interactions between the derivatives and tubulin were verified by fluorescence microscopy of living cells.

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