2. Academic Validation
  3. Physical and functional interactions between hematopoietic cell-specific ETS transcription factors and homeodomain proteins

Physical and functional interactions between hematopoietic cell-specific ETS transcription factors and homeodomain proteins

  • Leuk Res. 2009 Mar;33(3):483-9. doi: 10.1016/j.leukres.2008.07.002.
Toshiyuki Yamada 1,  Takeshi Shimizu,  Takuya Sakurai,  Naoki Nanashima,  Fumiko Kihara-Negishi,  Mitsuhiro Suzuki,  Yang Fan,  Miki Akita,  Tsuneyuki Oikawa,  Shigeki Tsuchida
Affiliations

Affiliation

  • 1 Department of Biochemistry and Genome Biology, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan.
PMID: 18692240 DOI: 10.1016/j.leukres.2008.07.002
Abstract

To examine the possibility that ETS family transcription factors, PU.1, SPI-B, ELF-1, ERG-3, ETS-1 and TEL, and homeodomain proteins, HOXA10, HOXC13, MEIS1 and PBX1B, function cooperatively, we investigated their interactions. In luciferase assays, HOXA10 and HOXC13 augmented the activity of PU.1 and SPI-B while diminishing that of ELF-1 and ERG-3. MEIS1 diminished the activity of ETS-1. No clear effects were observed for other combinations. Immunoprecipitation assays showed protein-protein interactions among the combinations exhibiting functional interactions. A mutation of HOXC13, which abolished binding to ELF-1, also abolished the diminishing effect on ELF-1. The results suggest functional interaction through physical interactions.

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