2. Academic Validation
  3. Enzymatic synthesis of peptides on a solid support

Enzymatic synthesis of peptides on a solid support

  • Org Biomol Chem. 2009 Feb 21;7(4):665-70. doi: 10.1039/b816847d.
Rose Haddoub 1,  Martin Dauner,  Fiona A Stefanowicz,  Valeria Barattini,  Nicolas Laurent,  Sabine L Flitsch
Affiliations

Affiliation

  • 1 Manchester Interdisciplinary Biocentre, The University of Manchester and School of Chemistry, 131 Princess Street, Manchester, UK M1 7DN.
PMID: 19194581 DOI: 10.1039/b816847d
Abstract

We have previously shown that dipeptides can be synthesised in high yields from Amino acids using protease catalysis in aqueous media, if the amino component is immobilised on porous PEGA resin (a copolymer of polyethylene glycol and polyacrylamide). Here we explore the scope of this methodology for using protected and glycosylated Amino acids as well as the synthesis of longer Peptides on resin and show that such a method can also be applied on non-porous surfaces, in particular on gold.

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