2. Academic Validation
  3. Induction of cell cycle arrest by the carbazole alkaloid Clauszoline-I from Clausena vestita D. D. Tao via inhibition of the PKCδ phosphorylation

Induction of cell cycle arrest by the carbazole alkaloid Clauszoline-I from Clausena vestita D. D. Tao via inhibition of the PKCδ phosphorylation

  • Eur J Med Chem. 2012 Jan;47(1):214-20. doi: 10.1016/j.ejmech.2011.10.047.
Wei Lin 1,  Ying Wang,  Sisi Lin,  Cuixian Li,  Chun Zhou,  Shaogui Wang,  Heqing Huang,  Peiqing Liu,  Guan Ye,  Xiaoyan Shen
Affiliations

Affiliation

  • 1 Laboratory of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, PR China.
PMID: 22093759 DOI: 10.1016/j.ejmech.2011.10.047
Abstract

Sixteen Carbazole Alkaloids from Clausena vestita D. D. Tao were extracted, and their anti-tumor activities were evaluated. Among the extracts, Clauszoline-I exhibited an obvious growth inhibitory activity against several Cancer cell lines through its ability to induce cell cycle arrest in the S and G2/M phases. A dramatic morphologic change with decreased F-actin staining and RhoA activity was found in Clauszoline-I treated HepG2 cells, in which the phosphorylation of PKCδ (Ser643) was inhibited. Our results indicated that induction cell cycle arrest by Clauszoline-I might be achieved by decreasing the RhoA activity via the inhibition of PKCδ phosphorylation.

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