2. Academic Validation
  3. Immunogenicity and bioactivity of glucagon, modified at methionine-27

Immunogenicity and bioactivity of glucagon, modified at methionine-27

  • Horm Metab Res. 1979 Aug;11(8):459-63. doi: 10.1055/s-0028-1092761.
W J Nooijen,  H J Kempen
PMID: 227776 DOI: 10.1055/s-0028-1092761
Abstract

Porcine glucagon was modified at methionine-27 by methylation or oxidation. Antisera against the glucagon derivatives were obtained. One of these antisera showed a high affinity for glucagon, with no cross-reactivity with gut-GLI 1. Biological activities of these derivatives were assessed on rat hepatocytes. Both derivatives had the same maximal glucose-mobilising activity as native glucagon, but a decrease potency, suggesting a crucial role of methionine in the binding of glucagon to its hepatic receptor.

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