2. Academic Validation
  3. Suppressive effects of coumarins from Mammea siamensis on inducible nitric oxide synthase expression in RAW264.7 cells

Suppressive effects of coumarins from Mammea siamensis on inducible nitric oxide synthase expression in RAW264.7 cells

  • Bioorg Med Chem. 2012 Aug 15;20(16):4968-77. doi: 10.1016/j.bmc.2012.06.031.
Toshio Morikawa 1,  Mayumi Sueyoshi,  Saowanee Chaipech,  Hisashi Matsuda,  Yukiko Nomura,  Mikuko Yabe,  Tomoko Matsumoto,  Kiyofumi Ninomiya,  Masayuki Yoshikawa,  Yutana Pongpiriyadacha,  Takao Hayakawa,  Osamu Muraoka
Affiliations

Affiliation

  • 1 Pharmaceutical Research and Technology Institute, Kinki University, 3-4-1 Kowakae, Higashi-osaka, Osaka 577-8502, Japan.
PMID: 22831798 DOI: 10.1016/j.bmc.2012.06.031
Abstract

A methanol extract of the flowers of Mammea siamensis (Calophyllaceae) was found to inhibit nitric oxide (NO) production in lipopolysaccharide-activated RAW264.7 cells. From the extract, two new geranylated Coumarins, mammeasins A (1) and B (2), were isolated together with 17 known compounds including 15 Coumarins. The structures of 1 and 2 were determined on the basis of their spectroscopic properties as well as of their chemical evidence. Among the isolates, 1 (IC(50) = 1.8 μM), 2 (6.4 μM), surangins B (3, 5.0 μM), C (4, 6.8 μM), and D (5, 6.2 μM), kayeassamins E (7, 6.1 μM), F (8, 6.0 μM), and G (9, 0.8 μM), mammea A/AD (11, 1.3 μM), and mammea E/BB (16, 7.9 μM) showed NO production inhibitory activity. Compounds 1, 9, and 11 were found to inhibit induction of inducible nitric oxide synthase (iNOS). With regard to mechanism of action of these active constituents (1, 9, and 11), suppression of STAT1 activation is suggested to be mainly involved in their suppression of iNOS induction.

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