1. Academic Validation
  2. Limonoids from the fruits of Melia azedarach and their cytotoxic activities

Limonoids from the fruits of Melia azedarach and their cytotoxic activities

  • Phytochemistry. 2013 May;89:59-70. doi: 10.1016/j.phytochem.2013.01.015.
Toshihiro Akihisa 1 Xin Pan Yasuhiro Nakamura Takashi Kikuchi Nami Takahashi Masahiro Matsumoto Eri Ogihara Makoto Fukatsu Kazuo Koike Harukuni Tokuda
Affiliations

Affiliation

  • 1 College of Science and Technology, Nihon University, 1-8-14 Kanda Surugadai, Tokyo 101-8308, Japan. akihisa.toshihiro@nihon-u.ac.jp
Abstract

Thirty-one limonoids and one tirucallane-type triterpenoid were isolated from the fruits of Melia azedarach (Meliaceae). The structures of 14 of these isolated compounds were elucidated on the basis of spectroscopic analyses and comparison with literature. All of these compounds were evaluated for their cytotoxic activities against HL60, A549, AZ521, and SK-BR-3 human Cancer cell lines. Meliarachin C (IC50 0.65 μM) and 3-O-deacetyl-4'-demethyl-28-oxosalannin (IC50 2.8 μM) exhibited potent cytotoxic activity against HL60 cells, and this was demonstrated mainly due to the induction of Apoptosis by flow cytometry. Western blot analysis suggested that both compounds induced Apoptosis via both the mitochondrial and death receptor-mediated pathways. In addition, 25 compounds were evaluated for their inhibitory effects against the Epstein-Barr virus early antigen (EBV-EA) activation induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) in Raji cells.

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  • HY-N3151
    Natural Product