1. Academic Validation
  2. Angelicin regulates LPS-induced inflammation via inhibiting MAPK/NF-κB pathways

Angelicin regulates LPS-induced inflammation via inhibiting MAPK/NF-κB pathways

  • J Surg Res. 2013 Nov;185(1):300-9. doi: 10.1016/j.jss.2013.05.083.
Fang Liu 1 Guo-quan Sun Hua-yi Gao Rui-sheng Li Lanan-Wassy Soromou Na Chen Yan-hong Deng Hai-hua Feng
Affiliations

Affiliation

  • 1 Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, Jilin, China.
Abstract

Background: Angelicin is a furocoumarin found in Psoralea corylifolia L. fruit. The purpose of this study was to investigate the protective ability of angelicin against inflammation in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and LPS-induced in vivo acute lung injury model.

Materials and methods: The concentrations of tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 in the culture supernatants of RAW 264.7 cells were determined 24 h after LPS administration. ALI was induced by intratracheal instillation of LPS. Six hours after LPS inhalation, bronchoalveolar lavage fluid and lung tissue samples were obtained for enzyme-linked immunosorbent assay, histologic, and Western blotting analyses.

Results: The results showed that pretreatment with angelicin markedly downregulated TNF-α and IL-6 levels in vitro and in vivo, and significantly decreased the amount of inflammatory cells, lung wet-to-dry weight ratio, and myeloperoxidase activity in LPS-induced ALI mice. Furthermore, Western blotting analysis results demonstrated that angelicin blocked the phosphorylation of IκBα, NF-κBp65, p38 MAPK, and JNK in LPS-induced ALI.

Conclusions: These results suggest that angelicin was potentially advantageous to prevent inflammatory diseases by inhibiting NF-κB and MAPK pathways. Our data indicated that angelicin might be a potential new agent for prevention of inflammatory reactions and diseases in the clinic.

Keywords

Angelicin; Cytokines; Lipopolysaccharide; MAPK; NF-κB.

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