2. Academic Validation
  3. Molecular basis of tubulin transport within the cilium by IFT74 and IFT81

Molecular basis of tubulin transport within the cilium by IFT74 and IFT81

  • Science. 2013 Aug 30;341(6149):1009-12. doi: 10.1126/science.1240985.
Sagar Bhogaraju 1,  Lukas Cajanek,  Cécile Fort,  Thierry Blisnick,  Kristina Weber,  Michael Taschner,  Naoko Mizuno,  Stefan Lamla,  Philippe Bastin,  Erich A Nigg,  Esben Lorentzen
Affiliations

Affiliation

  • 1 Department of Structural Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.
PMID: 23990561 DOI: 10.1126/science.1240985
Abstract

Intraflagellar transport (IFT) of ciliary precursors such as tubulin from the cytoplasm to the ciliary tip is involved in the construction of the cilium, a hairlike organelle found on most eukaryotic cells. However, the molecular mechanisms of IFT are poorly understood. Here, we found that the two core IFT proteins IFT74 and IFT81 form a tubulin-binding module and mapped the interaction to a calponin homology domain of IFT81 and a highly basic domain in IFT74. Knockdown of IFT81 and rescue experiments with point mutants showed that tubulin binding by IFT81 was required for ciliogenesis in human cells.

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