1. Academic Validation
  2. Bioactive oleanane-type saponins from the rhizomes of Anemone taipaiensis

Bioactive oleanane-type saponins from the rhizomes of Anemone taipaiensis

  • Bioorg Med Chem Lett. 2013 Oct 15;23(20):5714-20. doi: 10.1016/j.bmcl.2013.08.006.
Xiao-Yang Wang 1 Hui Gao Wei Zhang Yuan Li Guang Cheng Xiao-Li Sun Hai-Feng Tang
Affiliations

Affiliation

  • 1 Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an, PR China.
Abstract

Investigation of the n-BuOH extract of the rhizomes of Anemone taipaiensis led to the isolation of five new oleanane-type triterpenoid saponins (1-5), together with seven known saponins (6-12). Their structures were determined by the extensive use of (1)D and (2)D NMR experiments along with ESIMS analyses and acid hydrolysis. The aglycone of 1, 2 and 4 was determined as siaresinolic acid, which was reported in this genus for the first time. The cytotoxicities of the saponins 1-12, prosapogenins 4a, 5a, 10a-12a and sapogenins siaresinolic acid (SA), oleanolic acid (OA), hederagenin (HE) were evaluated against five human Cancer cell lines, including HepG2, HL-60, A549, HeLa and U87MG. The monodesmosidic saponins 6-8, 5a, 10a-12a and sapogenins SA, OA, HE exhibited cytotoxic activity toward all Cancer cell lines, with IC50 values ranging from 2.25 to 57.28 μM. Remarkably, the bisdesmosidic saponins 1-4 and 9 showed selective cytotoxicity against the U87MG cells.

Keywords

Anemone taipaiensis; Cytotoxicity; Ranunculaceae; Triterpenoid saponin.

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