2. Academic Validation
  3. 3,5,7,3',4'-pentamethoxyflavone, a quercetin derivative protects DNA from oxidative challenges: potential mechanism of action

3,5,7,3',4'-pentamethoxyflavone, a quercetin derivative protects DNA from oxidative challenges: potential mechanism of action

  • J Photochem Photobiol B. 2014 Feb 5:131:96-103. doi: 10.1016/j.jphotobiol.2014.01.003.
Rekha Jakhar 1,  Souren Paul 1,  Young Rong Park 2,  Jaehong Han 2,  Sun Chul Kang 3
Affiliations

Affiliations

  • 1 Department of Biotechnology, Daegu University, Kyongsan, Kyongbok 712-714, Republic of Korea.
  • 2 Metalloenzyme Research Group and School of Biological Sciences, Chung-Ang University, Anseong 456-756, Republic of Korea.
  • 3 Department of Biotechnology, Daegu University, Kyongsan, Kyongbok 712-714, Republic of Korea. Electronic address: sckang@daegu.ac.kr.
PMID: 24514458 DOI: 10.1016/j.jphotobiol.2014.01.003
Abstract

DNA protection is one of the most important strategies in Cancer therapy. Since quercetin and its derivatives are found to be potent antioxidant agents, they are able to scavenge radicals significantly. Therefore, we focused on the DNA protection activity of 3,5,7,3',4'-pentamethoxyflavone (PMF), a quercetin derivative isolated from Kaemperia parviflora. Although, PMF was found to be a very poor antioxidant compound, still it could remarkably protect DNA from oxidative damage. DNA binding assay showed that PMF bound to the minor groove of DNA, which suggests a possible mechanism for its DNA protective effects. Cellular toxicity assay on RAW 264.7 macrophages showed this compound is very safe for therapeutic applications.

Keywords

Antioxidant; Comet assay; DNA binding; DNA damage; ROS.

Figures
Products