Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis

Nat Struct Mol Biol. 2014 Aug;21(8):696-703. doi: 10.1038/nsmb.2846.

Suk-Youl Park ¹, Jung-Eun Park ², Tae-Sung Kim ², Ju Hee Kim ³, Mi-Jeong Kwak ⁴, Bonsu Ku ³, Lan Tian ⁵, Ravichandran N Murugan ⁶, Mija Ahn ⁶, Shinobu Komiya ⁷, Hironobu Hojo ⁸, Nam-Hyung Kim ⁹, Bo Yeon Kim ¹⁰, Jeong K Bang ⁶, Raymond L Erikson ¹¹, Ki Won Lee ¹², Seung Jun Kim ¹³, Byung-Ha Oh ¹⁴, Wei Yang ⁵, Kyung S Lee ¹⁵

Affiliations

1 1] Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA. [2] Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Republic of Korea. [3].
2 1] Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA. [2].
3 1] Medical Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea. [2].
4 1] Department of Biological Sciences, KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea. [2].
5 Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA.
6 Division of Magnetic Resonance, Korea Basic Science Institute, Ochang, Republic of Korea.
7 Department of Applied Biochemistry, Tokai University, Kanagawa, Japan.
8 Institute for Protein Research, Osaka University, Osaka, Japan.
9 Department of Animal Sciences, Chungbuk National University, Cheongju, Republic of Korea.
10 Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang, Republic of Korea.
11 Biological Laboratories, Harvard University, Cambridge, Massachusetts, USA.
12 1] Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Republic of Korea. [2] Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
13 Medical Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
14 Department of Biological Sciences, KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.
15 Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.

PMID: 24997597 DOI: 10.1038/nsmb.2846

Abstract

Polo-like kinase 4 (PLK4) is a key regulator of centriole duplication, an event critical for the maintenance of genomic integrity. We show that PLK4 relocalizes from the inner Cep192 ring to the outer Cep152 ring as newly recruited Cep152 assembles around the Cep192-encircled daughter centriole. Crystal-structure analyses revealed that Cep192- and Cep152-derived Peptides bind the cryptic polo box (CPB) of PLK4 in opposite orientations and in a mutually exclusive manner. The Cep152 peptide bound to the CPB markedly better than did the Cep192 peptide and effectively 'snatched' the CPB away from a preformed CPB-Cep192 peptide complex. A cancer-associated Cep152 mutation impairing the PLK4 interaction induced defects in procentriole assembly and chromosome segregation. Thus, PLK4 is intricately regulated in time and space through ordered interactions with two distinct scaffolds, Cep192 and Cep152, and a failure in this process may lead to human Cancer.

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