1. Academic Validation
  2. In vitro antioxidant and anti-inflammatory activities of Radix Isatidis extract and bioaccessibility of six bioactive compounds after simulated gastro-intestinal digestion

In vitro antioxidant and anti-inflammatory activities of Radix Isatidis extract and bioaccessibility of six bioactive compounds after simulated gastro-intestinal digestion

  • J Ethnopharmacol. 2014 Nov 18;157:55-61. doi: 10.1016/j.jep.2014.09.005.
Ping Xiao 1 Hongzhi Huang 1 Jianwei Chen 1 Xiang Li 2
Affiliations

Affiliations

  • 1 College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
  • 2 College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China. Electronic address: lixiang_8182@163.com.
Abstract

Ethnopharmacological relevance: Radix Isatidis called "Ban-Lan-Gen" is one of the most commonly-used traditional Chinese medicines for Antiviral, anti-inflammatory, antioxidant and antipyretic purposes. Investigate the bioaccessibility of uridine, epigoitrin, adenosine, clemastanin B, indigoticoside A and isolariciresinol as well as the antioxidant and anti-inflammatory activities during an in vitro gastro-intestinal digestion of the Radix Isatidis extract (RIE).

Materials and methods: High performance liquid chromatography (HPLC) technique was adopted to determine the bioaccessibility of six bioactive compounds in RIE. Antioxidant activities of RIE in different digestive stages were determined by 1,1-Diphenyl-2-picrylhydrazyl (DPPH), superoxide anion and hydroxyl radical scavenging abilities. Anti-inflammatory activity was assayed by the inhibitions of inflammatory cytokines such as nitrous oxide (NO), prostaglandin E2 (PGE2) and tumor necrosis factor α(TNF-α) producted by lipopolysaccharide (LPS) stimulated RAW264.7 cells.

Results: The bioaccessibility of uridine, epigoitrin, adenosine, clemastanin B, indigoticoside A and isolariciresinol were 15.38%, 18.28%, 24.01%, 6.50%, 8.65% and 17.78%, respectively. Also, the digestion products still possessed certain antioxidant activities. The antioxidant activity was highly correlated with Lignans (clemastanin B, indigoticoside A and isolariciresino). The anti-inflammation activity of the three samples decreased in the order: IN sample (the solution that had diffused into the dialysis tubing)>Nondigested sample (RIE solution)>Gastric sample (post-gastric digestion)>OUT sample (material that remained in the gastro-intestinal tract).

Conclusions: Results obtained in this research reveal the amount of bioactive compounds from RIE that could be available for absorption in vivo. The antioxidant activity decreased significantly but the anti-inflammatory activity was enhanced in serum-available fraction after gastro-intestinal digestion in vitro. This study could provide a scientific basis for a deeper pharmacological activity study of Radix Isatidis and a simple method for pharmacodynamic material basis research.

Keywords

Adenosine (Pubchem CID: 60961); Anti-inflammatory; Antioxidant; Bioaccessibility; DPPH (Pubchem CID: 2735032); Epigoitrin (Pubchem CID: 3032313); Hydrochloric acid (Pubchem CID: 313); Isolariciresinol (Pubchem CID: 160521); Lipopolysaccharide (Pubchem CID: 11970143); Nicotinamide-adenine dinucleotid (Pubchem CID: 5893); Nitro blue tetrazolium (Pubchem CID: 9281); PGE(2) (Pubchem CID: 5280360); Radix Isatidis; Simulated gastro-intestinal digestion; Uridine (Pubchem CID: 6029); Vitamin C (Pubchem CID: 54678501).

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